Expression of tissue factor and tissue factor pathway inhibitors during ovulation in rats: a relevance to the ovarian hyperstimulation syndrome

Abstract Background Blood coagulation has been associated with ovulation and female infertility. In this study, the expression of tissue factor system was examined during in immature rats; correlation between ovarian hyperstimulation syndrome (OHSS) evaluated both rats human follicular fluids. Methods Ovaries were obtained at various times after chorionic gonadotropin (hCG) injection to investigate system. Expression levels factor, pathway inhibitor (Tfpi)-1 Tfpi-2 genes proteins determined by real-time quantitative polymerase chain reaction (qPCR), Western blot immunofluorescence analyses, respectively. also investigated ovaries OHSS-induced fluid infertile women. Results The preovulatory follicles stimulated hCG, reaching a maximum 6 h. Tissue expressed oocytes follicles. mRNA mainly increased hCG granulosa cells whereas Tfpi-1 decreased hCG. Human CG-stimulated inhibited progesterone receptor antagonist. increase proliferator-activated γ (PPARγ) Decreased detected rats. Interestingly, concentrations fluids women undergoing vitro fertilization correlated pregnancy but not OHSS. Conclusions Collectively, results indicate that is ovulatory process moreover, exists OHSS humans.